Vector synthesis for gene manipulation and gene therapy
TakBioTech specializes in crafting personalized DNA vector constructions, precisely tailored to meet your unique research requirements. This encompasses but is not limited to, customizing codon sequences and regulatory elements. Our vector cloning platform is not only cost-effective but also robust, ensuring a swift turnaround time. Beyond this, TakBioTech extends its capabilities to provide comprehensive services, including plasmid DNA preparation, virus packaging for both LVV and AAV, and mRNA production.
Our comprehensive AAV gene therapeutic product development encompasses plasmid manufacturing across AAV upstream and downstream process development, culminating in fill and finish services.
Our production systems, based on mammalian cell HEK293 and insect cell Sf9, offer flexibility tailored to meet the specific requirements of each client. Our company offers upstream process testing and development, aiding clients in optimizing various process parameters, including plasmid ratio, cell density, media, transfection reagents, pH, and more, to achieve the most effective upstream process conditions. Additionally, we deliver upstream process development services for both adherent and suspension production.
Our Services at TakBioTech include:
– Two production systems for mammalian cells HEK293 and insect cells SF9
– Adherent and levitation upstream process types
– Complete and robust analytical methods ensure the quality of drug substance and finished products
– Production for investigator-initiated trials
Lentivirus, a single-stranded RNA retrovirus, serves as a valuable gene transfer tool in cell and gene therapies due to its capacity to infect both dividing and non-dividing differentiated cells.
Traditionally, lentiviral vectors (LVVs) have been manufactured using an adherent cell production platform with serum. However, as an alternative, serum-free suspension cell systems have been developed.
At TakBioTech, we’ve pioneered the creation of a proprietary suspension cell line, HEK293TG2S, achieved through clonal screening, suspension adaptation, and viral vector production technologies. Embracing advanced technologies and a commitment to quality and design, we’ve introduced the HEK293T Lentiviral Vector production platform (LV-SMART™).
This platform has demonstrated success in over a dozen client projects, excelling in multiple CAR-T/TCR-T tests.
Our Services at TakBioTech include:
-LVV production for trials
– Proprietary suspension HEK293T platform with a chemically defined medium (LV-SMART™)
– Adherent HEK293T platform (cells cloned and screened)
– Dedicated process development and analytical development teams.
TakBioTech excels in delivering plasmid DNA preparation services, offering swift and cost-effective solutions for high-quality DNAs.
Our comprehensive plasmid preparation services cater to a wide range of clients, including scientific researchers and industrial partners, accommodating quantities from micrograms to grams.
Our service portfolio extends beyond research projects to encompass the production of GMP-like and GMP-grade plasmid DNAs suitable for preclinical and clinical applications. Committed to excellence, we take pride in meeting the diverse needs of our customers within the academic, biotech, and pharmaceutical communities.
We provide complimentary small quantities of plasmid DNAs, and further expand our offerings with a diverse range of high-quality options available for purchase. Choose from Miniprep, Midi prep, Maxiprep, Mega prep, and Giga prep to suit your specific needs.
